B-Cell Linker Antikörper (pTyr84) (Alexa Fluor 488)

Produktnummer ABIN1177038

Dieses Anti-B-Cell Linker-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von B-Cell Linker in ICS. Geeignet für Human und Maus. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für B-Cell Linker Antikörper (pTyr84) (Alexa Fluor 488) (ABIN1177038)

Target: B-Cell Linker (BLNK)

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser B-Cell Linker Antikörper ist konjugiert mit Alexa Fluor 488

Applikation: Intracellular Staining (ICS)

Klon: J117-1278

Produktdetails anti-B-Cell Linker Antikörper (Alexa Fluor 488)

Bindungsspezifität: pTyr84

Marke: BD Phosflow™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human BLNK Peptide

Isotyp: IgG2b kappa

Anwendungsinformationen

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

Referenzen für anti-B-Cell Linker Antikörper (Alexa Fluor 488) (ABIN1177038)

Chiu, Dalton, Ishiai, Kurosaki, Chan: "BLNK: molecular scaffolding through 'cis'-mediated organization of signaling proteins." in: The EMBO journal, Vol. 21, Issue 23, pp. 6461-72, (2002) (PubMed).

Details zu B-Cell Linker

Target: B-Cell Linker (BLNK)

Andere Bezeichnung: BLNK

Hintergrund: B cell activation is initiated by crosslinking the B cell receptor, which leads to activation of non-receptor protein tyrosine kinases (PTK), including Btk, Syk, and three Src kinases, Fyn, Lyn, and Blk. Activated PTKs then phosphorylate multiple cellular proteins involved in B lymphocyte signaling. Syk is responsible for the tyrosine phosphorylation of B cell linker protein (BLNK), a member of the SLP-76 family of adapter proteins. Phosphorylation of human BLNK at tyrosines 84, 178, and 189 (Y84, Y178, and Y189) creates docking sites for PLCg2, leading to the activation of downstream signaling pathways. The J117-1278 monoclonal antibody recognizes the phosphorylated Y84 of BLNK.

Pathways: BCR Signaling