B-Cell Linker Antikörper (pTyr84) (Alexa Fluor 647)

Produktnummer ABIN1177037

Produktdetails anti-B-Cell Linker Antikörper (Alexa Fluor 647)

Target: B-Cell Linker (BLNK)

Bindungsspezifität: pTyr84

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser B-Cell Linker Antikörper ist konjugiert mit Alexa Fluor 647

Applikation: Intracellular Staining (ICS)

Marke: BD Phosflow™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human BLNK Peptide

Klon: J117-1278

Isotyp: IgG2b kappa

Anwendungsinformationen

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Referenzen für anti-B-Cell Linker Antikörper (Alexa Fluor 647) (ABIN1177037)

Pighi, Gu, Dalai, Barbi, Parolini, Bertolaso, Pedron, Parisi, Ren, Cecconi, Chilosi, Menestrina, Zamò: "Phospho-proteomic analysis of mantle cell lymphoma cells suggests a pro-survival role of B-cell receptor signaling." in: Cellular oncology (Dordrecht), Vol. 34, Issue 2, pp. 141-53, (2011) (PubMed).

Espeli, Mancini, Breton, Poirier, Schiff: "Impaired B-cell development at the pre-BII-cell stage in galectin-1-deficient mice due to inefficient pre-BII/stromal cell interactions." in: Blood, Vol. 113, Issue 23, pp. 5878-86, (2009) (PubMed).

Details zu B-Cell Linker

Target: B-Cell Linker (BLNK)

Andere Bezeichnung: BLNK (BLNK Produkte)

Synonyme: BLNK antikoerper, blnk antikoerper, MGC147045 antikoerper, BASH antikoerper, Bca antikoerper, Ly-57 antikoerper, Ly57 antikoerper, Lyw-57 antikoerper, SLP-65 antikoerper, AGM4 antikoerper, BLNK-S antikoerper, LY57 antikoerper, SLP65 antikoerper, bca antikoerper, B-cell linker antikoerper, B cell linker antikoerper, BLNK antikoerper, blnk antikoerper, Blnk antikoerper

Hintergrund: B cell activation is initiated by crosslinking the B cell receptor, which leads to activation of non-receptor protein tyrosine kinases (PTK), including Btk, Syk, and three Src kinases, Fyn, Lyn, and Blk. Activated PTKs then phosphorylate multiple cellular proteins involved in B lymphocyte signaling. Syk is responsible for the tyrosine phosphorylation of B cell linker protein (BLNK), a member of the SLP-76 family of adapter proteins. Phosphorylation of human BLNK at tyrosines 84, 178, and 189 (Y84, Y178, and Y189) creates docking sites for PLCg2, leading to the activation of downstream signaling pathways. The J117-1278 monoclonal antibody recognizes the phosphorylated Y84 of BLNK.

Pathways: BCR Signaling