B-Cell Linker Antikörper (pTyr84) (PE)

Produktnummer ABIN1177036

Dieses Maus Monoklonal-Antikörper erkennt spezifisch B-Cell Linker in ICS. Er zeigt eine Reaktivität gegenüber Human und Maus und wurde in 1 Publikation erwähnt.

Kurzübersicht für B-Cell Linker Antikörper (pTyr84) (PE) (ABIN1177036)

Target: B-Cell Linker (BLNK)

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser B-Cell Linker Antikörper ist konjugiert mit PE

Applikation: Intracellular Staining (ICS)

Klon: J117-1278

Produktdetails anti-B-Cell Linker Antikörper (PE)

Bindungsspezifität: pTyr84

Marke: BD Phosflow™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human BLNK Peptide

Isotyp: IgG2b kappa

Anwendungsinformationen

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Referenzen für anti-B-Cell Linker Antikörper (PE) (ABIN1177036)

Espeli, Mancini, Breton, Poirier, Schiff: "Impaired B-cell development at the pre-BII-cell stage in galectin-1-deficient mice due to inefficient pre-BII/stromal cell interactions." in: Blood, Vol. 113, Issue 23, pp. 5878-86, (2009) (PubMed).

Details zu B-Cell Linker

Target: B-Cell Linker (BLNK)

Andere Bezeichnung: BLNK

Hintergrund: B cell activation is initiated by crosslinking the B cell receptor, which leads to activation of non-receptor protein tyrosine kinases (PTK), including Btk, Syk, and three Src kinases, Fyn, Lyn, and Blk. Activated PTKs then phosphorylate multiple cellular proteins involved in B lymphocyte signaling. Syk is responsible for the tyrosine phosphorylation of B cell linker protein (BLNK), a member of the SLP-76 family of adapter proteins. Phosphorylation of human BLNK at tyrosines 84, 178, and 189 (Y84, Y178, and Y189) creates docking sites for PLCg2, leading to the activation of downstream signaling pathways. The J117-1278 monoclonal antibody recognizes the phosphorylated Y84 of BLNK.

Pathways: BCR Signaling