ITGA4 Antikörper

Produktnummer ABIN1176851

Der Ratte Monoklonal Anti-ITGA4-Antikörper wurde für FACS, IHC, IP und BR validiert. Er ist geeignet, ITGA4 in Proben von Maus zu detektieren. Es sind 5+ Publikationen verfügbar.

Kurzübersicht für ITGA4 Antikörper (ABIN1176851)

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser ITGA4 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Blocking Reagent (BR)

Klon: R1-2

Produktdetails anti-ITGA4 Antikörper

Marke: BD Pharmingen™

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: AKR/Cum mouse spontaneous T lymphoma line TK1

Isotyp: IgG2b kappa

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Konservierungsmittel: Azide free

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Referenzen für anti-ITGA4 Antikörper (ABIN1176851)

Brocke, Piercy, Steinman, Weissman, Veromaa: "Antibodies to CD44 and integrin alpha4, but not L-selectin, prevent central nervous system inflammation and experimental encephalomyelitis by blocking secondary leukocyte recruitment." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 12, pp. 6896-901, (1999) (PubMed).

Chisholm, Williams, Lobb: "Monoclonal antibodies to the integrin alpha-4 subunit inhibit the murine contact hypersensitivity response." in: European journal of immunology, Vol. 23, Issue 3, pp. 682-8, (1993) (PubMed).

Ferguson, Kupper: "Antigen-independent processes in antigen-specific immunity. A role for alpha 4 integrin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 4, pp. 1172-82, (1993) (PubMed).

Kilshaw, Murant: "Expression and regulation of beta 7(beta p) integrins on mouse lymphocytes: relevance to the mucosal immune system." in: European journal of immunology, Vol. 21, Issue 10, pp. 2591-7, (1991) (PubMed).

Holzmann, McIntyre, Weissman: "Identification of a murine Peyer's patch--specific lymphocyte homing receptor as an integrin molecule with an alpha chain homologous to human VLA-4 alpha." in: Cell, Vol. 56, Issue 1, pp. 37-46, (1989) (PubMed).

Details zu ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Andere Bezeichnung: CD49d

Hintergrund: The R1-2 antibody reacts with the integrin alpha4 chain (CD49d), which is expressed as a heterdimer with either of two beta, beta1 or beta7 (also known as betap). The alpha4beta1 integrin (VLA-4, CD49d/CD29) is expressed on most peripheral lymphocytes, thymocytes, and monocytes, while the alpha4beta7 integrin (LPAM-1) is expressed on peripheral lymphocytes, but on only a small subset of thymocytes. These integrins mediate a variety of cell-cell and cell-matrix interactions, recognizing the ligands VCAM-1 (CD106) and fibronectin. There is evidence that levels of VLA-4 expression regulate the transendothelial migration of T lymphocytes into inflamed tissues. Integrin alpha4beta7 also preferentially binds to the mucosal vascular addressin, MAdCAM-1. The R1-2 antibody blocks some alpha4 integrin-mediated binding functions. In combination with mAb 9C10 (MFR4.B) (Cat. No. 553313), binding of VLA-4 expressing cells to VCAM-1 can be almost completely inhibited.
Synonyms: Integrin alpha4 chain

Pathways: Integrin Complex