HMGB1 Antikörper

Produktnummer ABIN1176834

Dieses Anti-HMGB1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von HMGB1 in WB, IHC, IF, Inhibition und CA. Geeignet für Säugetier. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für HMGB1 Antikörper (ABIN1176834)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reaktivität: Säugetier

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HMGB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Blocking Antibody (Inhibition), Cellular Assay (CA)

Klon: DPH1-1

Produktdetails anti-HMGB1 Antikörper

Immunogen: 17-mer peptide KGKPDAAKKGVVKAEKS

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB: 1 µg/ml antibody in 5% milk TBS-T overnight at 4°C. Migration assay: 0.5/1/2/5 µg/ml of DPH1.1 mAb is added to 30 ng/ml of rHMGB1, according to Palumbo et al. “Extracellular HMGB1, a signal of tissue damage, induces mesoangioblast migration and proliferation”.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Handhabung: DPH1.1 mAb is provided freeze-dried.

Lagerung: -20 °C

Referenzen für anti-HMGB1 Antikörper (ABIN1176834)

Han, Yuan, Deng, He, Zhang, Shen, Chen, Qian: "Metformin decreases LPS-induced inflammatory response in rabbit annulus fibrosus stem/progenitor cells by blocking HMGB1 release." in: Aging, Vol. 11, Issue 22, pp. 10252-10265, (2020) (PubMed).

Details zu HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Andere Bezeichnung: High-mobility group protein B1 (HMGB1)

Hintergrund: DPH1.1 mAb recognizes all mammalian HMGB1s, including human, mouse and rat. Does not recognize HMGB2. DPH1.1 mAb can be used for Western blot (WB), immunofluorescence (IHF) and immunohistochemistry (IHC). DPH1.1 mAb blocks HMGB1-elicited cell migration in trans-well migration assays. In vivo, DPH1.1 mAb administered intravenously (220 µg/mouse) blocks recruitment of inflammatory cells to sites of necrosis and infection. The mouse monoclonal IgG1 DPH1.1 was generated by injecting C57BL/6 mice with the 17-mer peptide KGKPDAAKKGVVKAEKS. Hybridomas were produced from splenocytes by standard techniques and tested by ELISA against the immunogen and full-length HMGB1.

Pathways: p53 Signalweg, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome