LCAT Antikörper (AA 290-433) (Biotin)

Produktnummer ABIN1176764

Produktdetails anti-LCAT Antikörper (Biotin)

Target: LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))

Bindungsspezifität: AA 290-433

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LCAT Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Sequenz: MGHHHHHHSG SEF-I STPSFNYTGR DFQRFFADLH FEEGWYMWLQ SRDLLAGLPA PGVEVYCLYG VGLPTPRTYI YDHGFPYTDP VGVLYEDGDD TVATRSTELC GLWQGRQPQP VHLLPLHGIQ HLNMVFSNLT LEHINAILLG AYRQGPPASP TAS

Spezifität: It has been selected for its ability to recognize LCAT in immunohistochemical staining and Western blotting.

Aufreinigung: Affinity Chromatography

Immunogen: Recombinant LCAT expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against LCAT conjugated to biotin.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Kommentare:

Content: The quality control contains recombinant LCAT (Ile290~Ser433) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze/thaw cycles

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Haltbarkeit: 12 months

Details zu LCAT

Target: LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))

Andere Bezeichnung: Lecithin Cholesterol Acyltransferase (LCAT) (LCAT Produkte)

Synonyme: AI046659 antikoerper, D8Wsu61e antikoerper, MGC82035 antikoerper, lcat antikoerper, MGC88964 antikoerper, LCAT antikoerper, lecithin-cholesterol acyltransferase antikoerper, lecithin cholesterol acyltransferase antikoerper, lecithin-cholesterol acyltransferase L homeolog antikoerper, solute carrier family 12 member 4 antikoerper, fragile site, aphidicolin type, common, fra(13)(q13.2) antikoerper, LCAT antikoerper, Lcat antikoerper, lcat.L antikoerper, lcat antikoerper, SLC12A4 antikoerper, FRA13A antikoerper

Pathways: Lipid Metabolism