LCAT Antikörper (AA 290-433) (Biotin)

Produktnummer ABIN1176764

Der Kaninchen Polyklonal Anti-LCAT-Antikörper wurde für WB, IHC und ICC validiert. Er ist geeignet, LCAT in Proben von Human zu detektieren.

Kurzübersicht für LCAT Antikörper (AA 290-433) (Biotin) (ABIN1176764)

Target: LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LCAT Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Produktdetails anti-LCAT Antikörper (Biotin)

Bindungsspezifität: AA 290-433

Verwendungszweck: Biotin-Linked Polyclonal Antibody to Lecithin Cholesterol Acyltransferase (LCAT)

Sequenz: MGHHHHHHSG SEF-I STPSFNYTGR DFQRFFADLH FEEGWYMWLQ SRDLLAGLPA PGVEVYCLYG VGLPTPRTYI YDHGFPYTDP VGVLYEDGDD TVATRSTELC GLWQGRQPQP VHLLPLHGIQ HLNMVFSNLT LEHINAILLG AYRQGPPASP TAS

Spezifität: The antibody is a rabbit polyclonal antibody raised against LCAT. It has been selected for its ability to recognize LCAT in immunohistochemical staining and western blotting.

Kreuzreaktivität: Maus, Ratte

Aufreinigung: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant LCAT expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against LCAT conjugated to biotin.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze/thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu LCAT

Target: LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))

Andere Bezeichnung: Lecithin Cholesterol Acyltransferase

Hintergrund: Phosphatidylcholine-sterol O-acyltransferase, Phospholipid-cholesterol acyltransferase

Pathways: Lipid Metabolism