SFRP5 Antikörper (AA 45-162) (Biotin)

Produktnummer ABIN1175833

Produktdetails anti-SFRP5 Antikörper (Biotin)

Target: SFRP5 (Secreted Frizzled-Related Protein 5 (SFRP5))

Bindungsspezifität: AA 45-162

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SFRP5 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Sequenz: MGHHHHHHSG SEF-SKPPQC LDIPADLPLC HTVGYKRMRL PNLLEHESLA EVKQQASSWL PLLAKRCHSD TQVFLCSLFA PVCLDRPIYP CRSLCEAVRA GCAPLMEAYG FPWPEMLHCH KFPLDNDLCI AV

Spezifität: It has been selected for its ability to recognize SFRP5 in immunohistochemical staining and Western blotting.

Aufreinigung: Affinity Chromatography

Immunogen: Recombinant SFRP5 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against SFRP5 conjugated to biotin.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Kommentare:

Content: The quality control contains recombinant SFRP5 (Ser45~Val162) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze/thaw cycles

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Haltbarkeit: 12 months

Details zu SFRP5

Target: SFRP5 (Secreted Frizzled-Related Protein 5 (SFRP5))

Andere Bezeichnung: Secreted Frizzled Related Protein 5 (SFRP5) (SFRP5 Produkte)

Synonyme: SARP3 antikoerper, AI605071 antikoerper, D144 antikoerper, frp1 antikoerper, zgc:101849 antikoerper, Sfrp5 antikoerper, XsFRP5 antikoerper, frp5 antikoerper, sFRP-5 antikoerper, sarp3 antikoerper, sfrp5 antikoerper, sfrp5-a antikoerper, sfrp5-b antikoerper, secreted frizzled related protein 5 antikoerper, secreted frizzled-related sequence protein 5 antikoerper, secreted frizzled-related protein 5 antikoerper, secreted frizzled-related protein 5 L homeolog antikoerper, secreted frizzled-related protein 5 S homeolog antikoerper, SFRP5 antikoerper, Sfrp5 antikoerper, sfrp5 antikoerper, sfrp5.L antikoerper, sfrp5.S antikoerper

Pathways: WNT Signalweg