BDNF Antikörper (AA 129-247) (Biotin)

Produktnummer ABIN1171949

Der Kaninchen Polyklonal Anti-BDNF-Antikörper wurde für WB, IHC und ICC validiert. Er ist geeignet, BDNF in Proben von Human zu detektieren.

Kurzübersicht für BDNF Antikörper (AA 129-247) (Biotin) (ABIN1171949)

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BDNF Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Produktdetails anti-BDNF Antikörper (Biotin)

Bindungsspezifität: AA 129-247

Verwendungszweck: Biotin-Linked Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)

Sequenz: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GGPLGSPEF- HS DPARRGELSV CDSISEWVTA ADKKTAVDMS GGTVTVLEKV PVSKGQLKQY FYETKCNPMG YTKEGCRGID KRHWNSQCRT TQSYVRALTM DSKKRIGWRF IRIDTSCVCT LTIKRGR

Spezifität: The antibody is a rabbit polyclonal antibody raised against BDNF. It has been selected for its ability to recognize BDNF in immunohistochemical staining and western blotting.

Kreuzreaktivität: Maus, Ratte

Aufreinigung: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: PAA011Hu01Polyclonal Antibody to Brain Derived Neurotrophic Factor (BDNF)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze/thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu BDNF

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Andere Bezeichnung: Brain Derived Neurotrophic Factor

Hintergrund: Neurotrophin, Abrineurin

Pathways: RTK Signalweg, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity