Rekombinanter TUBB Antikörper

Produktnummer ABIN1169288

Der Human Monoklonal anti-TUBB Antikörper wird verwendet zum Nachweis von TUBB in Proben von Human, Maus, Ratte, Affe, Schwein und Drosophila melanogaster. Er wurde validiert für WB, ICC und ELISA.

Kurzübersicht für Rekombinanter TUBB Antikörper (ABIN1169288)

Target: TUBB (Tubulin, beta (TUBB))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Ratte, Affe, Schwein, Drosophila melanogaster

Wirt: Human

Klonalität: Monoklonal

Konjugat: Dieser TUBB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), ELISA

Klon: S11B

Produktdetails anti-TUBB Antikörper

Verwendungszweck: anti-beta-Tubulin, mAb (rec.) (S11B)

Spezifität: Recognizes human, mouse, rat, pig, drosophila and monkey beta-tubulin.

Produktmerkmale: Recombinant Antibody. Recognizes human, mouse, rat, pig, drosophila and monkey beta-tubulin. Species cross-reactivity: Drosophila, Human, Monkey, Mouse, Pig, Rat. Clone: S11B. Isotype: Human IgG2lambda. Applications: ELISA, ICC, WB. Host: Purified from HEK 293 cell culture supernatant. Liquid. In PBS containing 10 % glycerol and 0.02 % sodium azide. anti-beta-Tubulin, monoclonal antibody (recombinant)(S11B) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Aufreinigung: Purified from HEK 293 cell culture supernatant.

Reinheit: >95 % (SDS-PAGE)

Immunogen: Full length tubulin from pig brain.

Isotyp: IgG2 lambda

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

anti-beta-Tubulin, monoclonal antibody (recombinant) (S11B) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

+4°C

-20°C

Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.

Haltbarkeit: 12 months

Details zu TUBB

Target: TUBB (Tubulin, beta (TUBB))

Andere Bezeichnung: beta-Tubulin

Hintergrund:

Alternate Names/Synonyms: Tubulin beta Chain, Tubulin beta-5 chain

Product Description: anti-beta-Tubulin, monoclonal antibody (recombinant)(S11B) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Pathways: Microtubule Dynamics, M Phase