Rekombinanter TUBB Antikörper

Produktnummer ABIN1169288

Der Human Monoklonal Anti-TUBB-Antikörper wurde für WB, ELISA und ICC validiert. Er ist geeignet, TUBB in Proben von Human, Maus, Ratte, Schwein, Affe und Drosophila melanogaster zu detektieren.

Kurzübersicht für Rekombinanter TUBB Antikörper (ABIN1169288)

Target: TUBB (Tubulin, beta (TUBB))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Ratte, Schwein, Affe, Drosophila melanogaster

Wirt: Human

Klonalität: Monoklonal

Konjugat: Dieser TUBB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Klon: S11B

Produktdetails anti-TUBB Antikörper

Spezifität: Recognizes human, mouse, rat, pig, drosophila and monkey beta-tubulin.

Kreuzreaktivität: Fruchtfliege (Drosophila melanogaster), Human, Affe, Maus, Schwein, Ratte (Rattus)

Aufreinigung: Purified from HEK 293 cell culture supernatant.

Reinheit: >95 % (SDS-PAGE)

Immunogen: Full length tubulin from pig brain.

Isotyp: IgG2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

anti-beta-Tubulin, monoclonal antibody (recombinant) (S11B) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.

Haltbarkeit: 12 months

Details zu TUBB

Target: TUBB (Tubulin, beta (TUBB))

Andere Bezeichnung: beta-Tubulin

Hintergrund: Anti-beta-Tubulin, monoclonal antibody (recombinant)(S11B) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Pathways: Microtubule Dynamics, M Phase