Rekombinanter HMGB1 Antikörper

Produktnummer ABIN1169270

Der Human Monoklonal Anti-HMGB1-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, HMGB1 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für Rekombinanter HMGB1 Antikörper (ABIN1169270)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Ratte

Wirt: Human

Klonalität: Monoklonal

Konjugat: Dieser HMGB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Klon: Giby-1-4

Produktdetails anti-HMGB1 Antikörper

Spezifität: Recognizes human, mouse and rat HMGB1.

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Aufreinigung: Purified from HEK 293 cell culture supernatant.

Reinheit: >95 % (SDS-PAGE)

Immunogen: Human recombinant HMGB1.

Isotyp: IgG2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

HMGB1, monoclonal antibody (recombinant) (Giby1-4) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.

Haltbarkeit: 12 months

Details zu HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Andere Bezeichnung: HMGB1

Hintergrund: HMGB1 was originally discovered as an essential DNA-binding protein for regulating p53, NF-kappaB and other important proteins. It is secreted from activated dentric cells, macrophage and nectrotic cells, and acts as a ligand for RAGE, TLR-2 and TLR-4 expressed on surrounding cells. As a result, HMGB1 activates Rac, CDC42 and NF-kappaB inducing localized innate immunity of damaged tissue, tissue regeneration by recruitment of stem cells and hemostasis by induction of tissue factor expression. HMGB1 is also a causative agent of various diseases as it causes localized inflammation such as arteriosclerosis, chronic rheumatoid arthritis and nephritis. Anti-HMGB1, mAb (recombinant) (Giby-1-4) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

UniProt: P09429

Pathways: p53 Signalweg, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome