HCV 1b NS5B Antikörper

Produktnummer ABIN1169141

Es sind 6+ Publikationen für dieses Produkt verfügbar. Der Maus Monoklonal anti-HCV 1b NS5B Antikörper wird verwendet zum Nachweis von HCV 1b NS5B in Proben von Hepatitis C Virus (HCV). Er wurde validiert für WB.

Kurzübersicht für HCV 1b NS5B Antikörper (ABIN1169141)

Target: HCV 1b NS5B (HCV NS5b) (Hepatitis C Virus NS5b (HCV NS5b))

Reaktivität: Hepatitis C Virus (HCV)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HCV 1b NS5B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: 5B-3B1

Produktdetails anti-HCV 1b NS5B Antikörper

Verwendungszweck: anti-NS5B (HCV), mAb (5B-3B1)

Spezifität: Recognizes HCV NS5B (epitope mapped to NS5B aa 372-382). It recognizes genotype 1 a and also 1b (albeit with lower affinity). It does not recognize genotype 2a.

Kreuzreaktivität (Details): It does not recognize genotype 2a.

Produktmerkmale: Monoclonal Antibody. Recognizes HCV NS5B (epitope mapped to NS5B aa 372-382). It recognizes genotype 1 a and also 1b (albeit with lower affinity). It does not recognize genotype 2a. Isotype: Mouse IgG2b. Clone: 5B-3B1. Applications: WB. Liquid. In PBS containing 10 % glycerol and 0.02 % sodium azide. The NS3 serine protease, in association with NS4A, is responsible for the cleavages of the Hepatitis C virus protease complexes NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Hepatitis C virus protease NS3-NS4A colocalizes with IPS-1/VISA/MAVS/Cardif in the mitochondrial membrane and has been shown to cleave the TLR3 adapter TRIF and Cardif to eliminate the antiviral signaling pathways. NS3-NS4A represents an important target for anti-HCV drugs.

Aufreinigung: Purified from concentrated hybridoma tissue culture supernatant.

Reinheit: >95 % (SDS-PAGE)

Immunogen: Recombinant hepatitis C virus (HCV) NS5B RNA-dependent RNA polymerase (genotype 1a).

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

+4°C

-20°C

Stable for at least 1 year after receipt when stored at -20°C.

Haltbarkeit: 12 months

Referenzen für anti-HCV 1b NS5B Antikörper (ABIN1169141)

Kapadia, Chisari: "Hepatitis C virus RNA replication is regulated by host geranylgeranylation and fatty acids." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 7, pp. 2561-6, (2005) (PubMed).

Quinkert, Bartenschlager, Lohmann: "Quantitative analysis of the hepatitis C virus replication complex." in: Journal of virology, Vol. 79, Issue 21, pp. 13594-605, (2005) (PubMed).

Svitkin, Pause, Lopez-Lastra, Perreault, Sonenberg: "Complete translation of the hepatitis C virus genome in vitro: membranes play a critical role in the maturation of all virus proteins except for NS3." in: Journal of virology, Vol. 79, Issue 11, pp. 6868-81, (2005) (PubMed).

Hardy, Marcotrigiano, Blight, Majors, Rice: "Hepatitis C virus RNA synthesis in a cell-free system isolated from replicon-containing hepatoma cells." in: Journal of virology, Vol. 77, Issue 3, pp. 2029-37, (2003) (PubMed).

Randall, Grakoui, Rice: "Clearance of replicating hepatitis C virus replicon RNAs in cell culture by small interfering RNAs." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 1, pp. 235-40, (2003) (PubMed).

Moradpour, Bieck, Hügle, Wels, Wu, Hong, Blum, Bartenschlager: "Functional properties of a monoclonal antibody inhibiting the hepatitis C virus RNA-dependent RNA polymerase." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 593-601, (2002) (PubMed).

Details zu HCV 1b NS5B

Target: HCV 1b NS5B (HCV NS5b) (Hepatitis C Virus NS5b (HCV NS5b))

Substanzklasse: Viral Protein

Hintergrund:

Alternate Names/Synonyms: Nonstructural Protein 5B

Product Description: The NS3 serine protease, in association with NS4A, is responsible for the cleavages of the Hepatitis C virus protease complexes NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Hepatitis C virus protease NS3-NS4A colocalizes with IPS-1/VISA/MAVS/Cardif in the mitochondrial membrane and has been shown to cleave the TLR3 adapter TRIF and Cardif to eliminate the antiviral signaling pathways. NS3-NS4A represents an important target for anti-HCV drugs.