BTLA Antikörper

Produktnummer ABIN1169098

Dieser Ratte Monoklonal Antikörper detektiert spezifisch BTLA in FACS und ELISA. Es zeigt Reaktivität gegenüber Proben von Human. Es wurde in 2+ Publikationen zitiert.

Kurzübersicht für BTLA Antikörper (ABIN1169098)

Target: BTLA (B and T Lymphocyte Associated (BTLA))

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser BTLA Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), ELISA

Klon: 6F4

Produktdetails anti-BTLA Antikörper

Verwendungszweck: anti-BTLA (human), mAb (6F4)

Spezifität: Recognizes human BTLA.

Produktmerkmale: Monoclonal Antibody. Recognizes human BTLA. Isotype: Rat IgG1. Clone: 6F4. Applications: ELISA, FACS, FUNC (Blocking). Liquid. In PBS containing 10 % glycerol. B and T lymphocyte associated (BTLA) is an Ig domain superfamily protein with cytoplasmic immunoreceptor tyrosine-based inhibitory motifs. The herpes virus entry mediator (HVEM), a member of the TNF receptor (TNFR) superfamily, can act as a molecular switch that modulates T cell activation by propagating positive signals from the TNF-related ligand LIGHT (TNFR superfamily 14), or inhibitory signals through the Ig superfamily member BTLA. The binding site on HVEM for BTLA is conserved in the orphan TNF receptor UL144, present in human CMV. UL144 binds BTLA, but not LIGHT, and inhibits T cell proliferation, selectively mimicking the inhibitory cosignaling function of HVEM.

Aufreinigung: Purified from concentrated hybridoma tissue culture supernatant.

Reinheit: >95 % (SDS-PAGE)

Endotoxin-Niveau: <0.001EU/μg purified protein.

Immunogen: Ectodomain of human BTLA fused to human IgG Fc.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: In PBS containing 10 % glycerol.

Handhabung: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

+4°C

-20°C

Stable for at least 1 year after receipt when stored at -20°C.

Haltbarkeit: 12 months

Referenzen für anti-BTLA Antikörper (ABIN1169098)

Cheung, Oborne, Steinberg, Macauley, Fukuyama, Sanjo, DSouza, Norris, Pfeffer, Murphy, Kronenberg, Spear, Ware: "T cell intrinsic heterodimeric complexes between HVEM and BTLA determine receptivity to the surrounding microenvironment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 11, pp. 7286-96, (2009) (PubMed).

Cheung, Steinberg, Oborne, Macauley, Fukuyama, Sanjo, DSouza, Norris, Pfeffer, Murphy, Kronenberg, Spear, Ware: "Unconventional ligand activation of herpesvirus entry mediator signals cell survival." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 15, pp. 6244-9, (2009) (PubMed).

Details zu BTLA

Target: BTLA (B and T Lymphocyte Associated (BTLA))

Andere Bezeichnung: BTLA

Hintergrund:

Alternate Names/Synonyms: B and T Lymphocyte Associated, Lymphotoxin-beta Receptor, Tumor Necrosis Factor Receptor 2 Related Protein, Tumor Necrosis Factor C Receptor, Tumor Necrosis Factor Receptor Superfamily Member 3, TNFRSF3

Product Description: B and T lymphocyte associated (BTLA) is an Ig domain superfamily protein with cytoplasmic immunoreceptor tyrosine-based inhibitory motifs. The herpes virus entry mediator (HVEM), a member of the TNF receptor (TNFR) superfamily, can act as a molecular switch that modulates T cell activation by propagating positive signals from the TNF-related ligand LIGHT (TNFR superfamily 14), or inhibitory signals through the Ig superfamily member BTLA. The binding site on HVEM for BTLA is conserved in the orphan TNF receptor UL144, present in human CMV. UL144 binds BTLA, but not LIGHT, and inhibits T cell proliferation, selectively mimicking the inhibitory function of HVEM. BTLA also interacts with HVEM in cis, forming a heterodimeric complex in naive T cells that inhibits HVEM-dependent signaling. The cis-interaction between HVEM and BTLA is the predominant form expressed on the surface of naive human and mouse T cells and may be important to limit trans-signaling by LIGHT, BTLA, and CD160 that are expressed in adjacent activated cells.

UniProt: Q7Z6A9

Pathways: Cancer Immune Checkpoints