alpha Tubulin Antikörper (TUBA1)

Details for Product anti-TUBA1 Antibody No. ABIN1169084, Anbieter: Anmelden zum Anzeigen
Antigen
  • Tuba1
  • K-ALPHA-1
  • TUBA2
  • Tuba-1
  • cb944
  • fb22g06
  • tuba1
  • wu:fb22g06
  • mec-12
  • tuba4
  • Calpha1
  • tubulin, alpha 1A
  • tubulin alpha 1b
  • tubulin, alpha 3e
  • tubulin, alpha 1b
  • tubulin alpha 4a
  • tubulin-alpha
  • tubulin alpha 4b L homeolog
  • tubulin, alpha 1B
  • Tuba1a
  • TUBA1B
  • TUBA3E
  • tuba1b
  • TUBA4A
  • LOC100101229
  • tuba4b.L
  • Tuba1b
Reaktivität
Rind (Kuh), Human, Maus
263
156
144
63
57
55
47
32
31
29
29
29
29
28
28
26
19
19
19
17
9
8
8
8
8
7
6
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Wirt
Human
165
111
26
5
2
2
1
1
Antikörpertyp
Recombinant Antibody
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser alpha Tubulin Antikörper ist unkonjugiert
15
11
8
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Western Blotting (WB)
272
104
93
92
91
91
57
39
35
13
11
8
4
4
2
2
2
2
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Immunogen Bovine brain tubulin.
Klon F2C
Isotyp IgG2
Spezifität Recognizes mouse, bovine and human alpha-tubulin.
Kreuzreaktivität Rind (Kuh), Human, Maus
Reinigung Purified from HEK 293 cell culture supernatant.
Reinheit >95 % (SDS-PAGE)
Andere Bezeichnung alpha-Tubulin (TUBA1 Antibody Abstract)
Hintergrund Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton.
Pathways Microtubule Dynamics
Applikationshinweise Optimal working dilution should be determined by the investigator.
Kommentare

anti-alpha-Tubulin, monoclonal antibody (recombinant) (F2C) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain. anti-alpha-Tubulin, monoclonal antibody (recombinant) (F2C) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer In PBS containing 10 % glycerol and 0.02 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.
Haltbarkeit 12 months
Produkt verwendet in: Vielemeyer, Nizak, Jimenez, Echard, Goud, Camonis, Rain, Perez: "Characterization of single chain antibody targets through yeast two hybrid." in: BMC biotechnology, Vol. 10, pp. 59, 2010 (PubMed).

Nizak, Martin-Lluesma, Moutel, Roux, Kreis, Goud, Perez: "Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo." in: Traffic (Copenhagen, Denmark), Vol. 4, Issue 11, pp. 739-53, 2003 (PubMed).

Haben Sie etwas anderes gesucht?