Rekombinanter alpha Tubulin Antikörper

Produktnummer ABIN1169084

Der Human Monoklonal Anti-alpha Tubulin-Antikörper wurde für WB und ICC validiert. Er ist geeignet, alpha Tubulin in Proben von Human, Maus und Rind (Kuh) zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für Rekombinanter alpha Tubulin Antikörper (ABIN1169084)

Target: alpha Tubulin (TUBA1)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Rind (Kuh)

Wirt: Human

Klonalität: Monoklonal

Konjugat: Dieser alpha Tubulin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC)

Klon: F2C

Produktdetails anti-alpha Tubulin Antikörper

Spezifität: Recognizes mouse, bovine and human alpha-tubulin.

Kreuzreaktivität: Rind (Kuh), Human, Maus

Aufreinigung: Purified from HEK 293 cell culture supernatant.

Reinheit: >95 % (SDS-PAGE)

Immunogen: Bovine brain tubulin.

Isotyp: IgG2

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

anti-alpha-Tubulin, monoclonal antibody (recombinant) (F2C) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain. anti-alpha-Tubulin, monoclonal antibody (recombinant) (F2C) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.

Haltbarkeit: 12 months

Referenzen für anti-alpha Tubulin Antikörper (ABIN1169084)

Vielemeyer, Nizak, Jimenez, Echard, Goud, Camonis, Rain, Perez: "Characterization of single chain antibody targets through yeast two hybrid." in: BMC biotechnology, Vol. 10, pp. 59, (2010) (PubMed).

Nizak, Martin-Lluesma, Moutel, Roux, Kreis, Goud, Perez: "Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo." in: Traffic (Copenhagen, Denmark), Vol. 4, Issue 11, pp. 739-53, (2003) (PubMed).

Details zu alpha Tubulin

Target: alpha Tubulin (TUBA1)

Andere Bezeichnung: alpha-Tubulin

Hintergrund: Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton.

Pathways: Microtubule Dynamics