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GLUD1 Antikörper

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch GLUD1 in WB und EIA. Er zeigt eine Reaktivität gegenüber Rind (Kuh).
Produktnummer ABIN116725

Kurzübersicht für GLUD1 Antikörper (ABIN116725)

Target

Alle GLUD1 Antikörper anzeigen
GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Reaktivität

  • 36
  • 20
  • 18
  • 17
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Rind (Kuh)

Wirt

  • 48
  • 6
  • 1
Kaninchen

Klonalität

  • 47
  • 8
Polyklonal

Konjugat

  • 39
  • 5
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser GLUD1 Antikörper ist unkonjugiert

Applikation

  • 41
  • 28
  • 19
  • 16
  • 15
  • 11
  • 6
  • 5
  • 5
  • 4
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Enzyme Immunoassay (EIA)
  • Spezifität

    Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glutamate Dehydrogenase [Bovine Liver]. BLAST analysis was used to determine that cross reactivity is suggested for both mitochondrial and brain isoforms (GDH1 and GDH2), from both bovine and human sources. Additionally similar reactivity is suggested for most primate species including green monkey, white gibbon, chimpanzee orangutan, and gorilla. A high degree of sequence homology is also noted for GDH from chicken, mouse, rat, dog, and other mammals as well as Xenopus tropicalis, zebrafish, rainbow trout and Atlantic salmon. Cross reactivity against Glutamate Dehydrogenase from other tissues and species may occur but have not been specifically determined.

    Aufreinigung

    Delipidation and defibrination.

    Immunogen

    A full length Glutamate Dehydrogenase protein isolated from Bovine Liver.
  • Applikationshinweise

    This antibody has been tested for use in ELISA and by Western blot. Bovine glutamatedehydrogenase exists as a homohexamer located within the mitochondrial matrix. Expect aband approximately 62 kDa in size corresponding to glutamate dehydrogenase monomersubunit by Western blotting in the appropriate cell or tissue extract. Recommended Dilutions: ELISA: 1/4,000-1/16,000Western blot: 1/1,000-1/3,000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Rekonstitution

    Restore with 2.0 mL of deionized water (or equivalent).

    Konzentration

    85.0 mg/mL (by refractometry)

    Buffer

    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

    Lagerung

    4 °C/-20 °C

    Informationen zur Lagerung

    Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below.
  • Target

    GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

    Andere Bezeichnung

    Glutamate Dehydrogenase 1

    Hintergrund

    Glutamate is a major excitatory neurotransmitter. One enzyme central to the metabolism of glutamate is glutamate dehydrogenase (GDH1, EC 1.4.1.3), that catalyzes the reversible deamination of L-glutamate to 2-oxoglutarate using NAD+ or NADP+. Mammalian GDH is composed of six identical subunits, and the regulation of GDH is very complex. It has been a major goal to identify the substrate and regulatory binding sites of GDH. It is only in recent years that the three-dimensional structure of GDH from microorganisms is available. Very recently, crystallization of bovine liver GDH was reported for the first time from the mammalian sources. However, remarkably little is known about the detailed structure of mammalian GDH, especially the brain enzymes.Synonyms: GDH1, GLUD, GLUD1

    Gen-ID

    2746

    NCBI Accession

    NP_005262

    UniProt

    P00367

    Pathways

    Positive Regulation of Peptide Hormone Secretion, Warburg Effekt
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