GLUD1 Antikörper

Produktnummer ABIN116725

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch GLUD1 in WB und EIA. Er zeigt eine Reaktivität gegenüber Rind (Kuh).

Kurzübersicht für GLUD1 Antikörper (ABIN116725)

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLUD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-GLUD1 Antikörper

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glutamate Dehydrogenase [Bovine Liver]. BLAST analysis was used to determine that cross reactivity is suggested for both mitochondrial and brain isoforms (GDH1 and GDH2), from both bovine and human sources. Additionally similar reactivity is suggested for most primate species including green monkey, white gibbon, chimpanzee orangutan, and gorilla. A high degree of sequence homology is also noted for GDH from chicken, mouse, rat, dog, and other mammals as well as Xenopus tropicalis, zebrafish, rainbow trout and Atlantic salmon. Cross reactivity against Glutamate Dehydrogenase from other tissues and species may occur but have not been specifically determined.

Aufreinigung: Delipidation and defibrination.

Immunogen: A full length Glutamate Dehydrogenase protein isolated from Bovine Liver.

Anwendungsinformationen

Applikationshinweise: This antibody has been tested for use in ELISA and by Western blot. Bovine glutamatedehydrogenase exists as a homohexamer located within the mitochondrial matrix. Expect aband approximately 62 kDa in size corresponding to glutamate dehydrogenase monomersubunit by Western blotting in the appropriate cell or tissue extract. Recommended Dilutions: ELISA: 1/4,000-1/16,000Western blot: 1/1,000-1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with 2.0 mL of deionized water (or equivalent).

Konzentration: 85.0 mg/mL (by refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below.

Details zu GLUD1

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Andere Bezeichnung: Glutamate Dehydrogenase 1

Hintergrund: Glutamate is a major excitatory neurotransmitter. One enzyme central to the metabolism of glutamate is glutamate dehydrogenase (GDH1, EC 1.4.1.3), that catalyzes the reversible deamination of L-glutamate to 2-oxoglutarate using NAD+ or NADP+. Mammalian GDH is composed of six identical subunits, and the regulation of GDH is very complex. It has been a major goal to identify the substrate and regulatory binding sites of GDH. It is only in recent years that the three-dimensional structure of GDH from microorganisms is available. Very recently, crystallization of bovine liver GDH was reported for the first time from the mammalian sources. However, remarkably little is known about the detailed structure of mammalian GDH, especially the brain enzymes.Synonyms: GDH1, GLUD, GLUD1

Gen-ID: 2746

NCBI Accession: NP_005262

UniProt: P00367

Pathways: Positive Regulation of Peptide Hormone Secretion, Warburg Effekt