GLB1 Antikörper

Produktnummer ABIN116640

Der Kaninchen Polyklonal anti-GLB1 Antikörper (ABIN116640) detektiert spezifisch GLB1 in WB, IHC (p), EIA und IHC (fro). Dieser Antikörper reagiert spezifisch mit Proben aus E. coli.

Kurzübersicht für GLB1 Antikörper (ABIN116640)

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Reaktivität: E. coli

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-GLB1 Antikörper

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Beta Galactosidase [E. coli]. Cross reactivity against Beta Galactosidase from other sources may occur but have not been specifically determined.

Aufreinigung: Purified from monospecific antiserum by Delipidation and Defribination.

Immunogen: Beta Galactosidase [E. coli].

Anwendungsinformationen

Applikationshinweise: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: ELISA: 1/65,000. Western blot: 1/500-1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with 2.0 mL of deionized water (or equivalent).

Konzentration: 80 mg/mL (by refractometry)

Buffer: 0.02 M Potassiumphosphate, 0.15 M Sodium chloride, pH 7.2 with 0.01 % Sodium azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C. For extended storage aliquot contents and freeze at -20 °C or below.

Details zu GLB1

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Andere Bezeichnung: beta Galactosidase (GAL)

Hintergrund: Beta-galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.Synonyms: Beta-Gal Fusion Protein, Beta-Gal tag, JW0335, Lactase, b0344, lacZ tag

Gen-ID: 945006

NCBI Accession: AP_000996

UniProt: P00722

Pathways: Glycosaminoglycan Metabolic Process