Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Adenosine Deaminase [Calf Spleen]. Cross reactivity against Adenosine Deaminase from other tissues and species are not specifically determined, but are very probable due to high sequence homologie (human >90 % , mouse/rat 85 %).
Aufreinigung
Prepared from monospecific antiserum by a delipidation and defibrination.
Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation,Conjugation and most immunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 0.3 μg of AdenosineDeaminase [Calf Spleen] by immunoblot using Peroxidase conjugated Affinity Purifiedanti-Rabbit IgG [H&L] (Goat) (R1364HRP) and DAB as a substrate at room temperature. Aworking dilution of 1: 5,000 to 1: 10,000 of the reconstitution concentration is suggested forthis product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
Restore with 2.0 mL of deionized water (or equivalent).
Konzentration
90.0 mg/mL (by Refractometry)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.