Clusterin Antikörper

Produktnummer ABIN116548

Dieser Ziege Polyklonal Antikörper detektiert spezifisch Clusterin in WB, IP, IHC (p), EIA und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Clusterin Antikörper (ABIN116548)

Target: Clusterin (CLU)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Clusterin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-Clusterin Antikörper

Spezifität: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1 % cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with apoLipoprotein J and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, C-III and E apoLipoproteins. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.

Aufreinigung: Immunoaffinity chromatography

Immunogen: ApoLipoprotein Type J was isolated from human plasma by density gradient centrifugation followed by HPLC purification.

Anwendungsinformationen

Applikationshinweise: Has been used for indirect trapping ELISA for quantitation of antigen in serum using astandard curve, for immunoprecipitation and for western blotting for highly sensitivequalitative analysis. Recommended dilutions: ELISA 1: 5,000 - 1: 10,000. Western blot 1: 5,000 - 1: 10,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, containing 0.01 % (w/v) Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: This product is stable at 2-8 °C as an undiluted liquid for up to one month. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20 °C or below.

Details zu Clusterin

Target: Clusterin (CLU)

Andere Bezeichnung: Apolipoprotein J (Apo J)

Hintergrund: At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individual's relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Apolipoprotein J is the major secreted product of Sertoli cells and is thought to play a critical role in spermatogenesis. The protein was shown to be a normal constituent of human blood and is also thought to be a control mechanism of the complement cascade. It prevents the binding of a C5b-C7 complex to the membrane of the target cell and in this way inhibits complement-mediated cytolysis. Apolipoprotein J is overexpressed in human prostate and breast cancers and in squamous cell carcinomas. Suppression of Apolipoprotein J renders these cells sensitive to chemotherapeutic drug-mediated apoptosis. It has been proposed that elevated levels of Apolipoprotein J may promote oncogenic transformation and tumor progression by interfering with BAX proapoptotic activities.Synonyms: 40, 40, AAG4, APOJ, Aging-associated gene 4 protein, CLI, Clusterin, Complement cytolysis inhibitor, Complement-associated protein SP-40, KUB1, Ku70-binding protein 1, NA1/NA2, SP-40, Testosterone-repressed prostate message 2

Gen-ID: 1191

NCBI Accession: NP_976084

UniProt: P10909

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling