APOE Antikörper

Produktnummer ABIN116547

Der Ziege Polyklonal anti-APOE Antikörper (ABIN116547) detektiert spezifisch APOE in WB, IP, IHC (p), IHC (fro) und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für APOE Antikörper (ABIN116547)

Target: APOE (Apolipoprotein E (APOE))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser APOE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Produktdetails anti-APOE Antikörper

Spezifität: This product has been prepared by Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other Apolipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1 % cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human Apolipoprotein E and has negligible cross-reactivity with Type A-1 A-II, B, C-I, C-II, C-III and J Apolipoproteins. Specific cross reaction of anti-Apolipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-Apolipoprotein antibodies with other human serum proteins is negligible.

Aufreinigung: Immunoaffinity Chromatography

Immunogen: Apolipoprotein Type E was isolated from Human plasma by density gradient centrifugation followed by HPLC purification.

Anwendungsinformationen

Applikationshinweise: Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitationof antigen in serum using a standard curve, for immunoprecipitation and for westernblotting for highly sensitive qualitative analysis. Recommended dilutions: ELISA: 1/4,000-1/8,000Western blot: 1/500-1/2,000Immunohistochemistry: 1/50-1/200
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, containing 0.01 % Sodium Azide as preservtive

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquts) at -20 °C for longer.

Details zu APOE

Target: APOE (Apolipoprotein E (APOE))

Andere Bezeichnung: Apolipoprotein E (Apo E)

Hintergrund: Apolipoprotein E is essential for the normal catabolism of triglyceride rich lipoprotein constituents. The apolipoprotein E gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in Apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Mutations in the APOE gene confer susceptibility to Alzheimer's disease by affecting amyloid-beta deposition.Synonyms: Apo-E, ApoE

Gen-ID: 348

NCBI Accession: NP_000032

UniProt: P02649

Pathways: Regulation of Cell Size, Lipid Metabolism