APOC3 Antikörper

Produktnummer ABIN116546

Der Ziege Polyklonal anti-APOC3 Antikörper wird verwendet zum Nachweis von APOC3 in Proben von Human. Er wurde validiert für WB, IP, IHC (p), IHC (fro) und EIA.

Kurzübersicht für APOC3 Antikörper (ABIN116546)

Target: APOC3 (Apolipoprotein C-III (APOC3))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser APOC3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Produktdetails anti-APOC3 Antikörper

Spezifität: This product has been prepared by Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1 % cross reactivity against other types of ApoLipoprotein was detected by ELISA against purified standards. This antibody recognizes Human ApoLipoprotein C-III and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, E and J ApoLipoproteins. Non-specific cross reaction of anti-ApoLipoprotein antibodies with other Human serum proteins is negligible.

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: Synthetic full-length Apolipoprotein Type C-III (not selected epitopes) using conventional peptide technology.

Anwendungsinformationen

Applikationshinweise: This Apolipoprotein antibody has been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for Immunoprecipitation, Western blotting forhighly sensitive qualitative analysis and for Immunohistochemistry. Recommended Dilutions: ELISA: 1/5,000-1/10,000. Western blot: 1/5,000-1/10,000. Immunoprecipitation: 1/100. Immunohistochemistry Paraffin Sections: 2.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, with 0.01 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at -20 °C.

Details zu APOC3

Target: APOC3 (Apolipoprotein C-III (APOC3))

Andere Bezeichnung: Apolipoprotein C III (Apo CIII)

Hintergrund: At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Apolipoprotein C-III is a very low density lipoprotein (VLDL) protein. It inhibits lipoprotein lipase and hepatic lipase and it is thought to delay catabolism of triglyceride-rich particles. An increase in ap °C-III levels induces the development of hypertriglyceridemia.Synonyms: Apo-CIII, Ap °C-III, Ap °C3, Apolipoprotein C-III, Apolipoprotein C3

Gen-ID: 345

NCBI Accession: NP_000031

UniProt: P02656

Pathways: Carbohydrate Homeostasis, Lipid Metabolism