APOA2 Antikörper

Produktnummer ABIN116542

Dieser Ziege Polyklonal Antikörper detektiert spezifisch APOA2 in WB, IP, IHC (p), EIA und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für APOA2 Antikörper (ABIN116542)

Target: APOA2 (Apolipoprotein A-II (APOA2))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser APOA2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-APOA2 Antikörper

Spezifität: This product has been prepared by Immunoaffinity Chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1 % cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with ApoLipoprotein A-II and has negligible cross-reactivity with Type A-I, B, C-I, C-II, C-III, E and J apoLipoproteins. Non-specific cross reaction of anti-apoLipoprotein antibodies with other Human serum proteins is negligible.

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: ApoLipoprotein Type A-II was isolated from Human plasma by Density Gradient Centrifugation followed by HPLC purification.

Anwendungsinformationen

Applikationshinweise: This Apo AII antibody R1031P has been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for Immunoprecipitation and Western blotting forhighly sensitive qualitative analysis. Recommended Dilutions: ELISA: 1/5,000-1/10,000. Immunoprecipitation: 1/100. Western blot: 1/5,000-1/10,000. Immunohistochemistry: 1: /50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 and 0.01 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu APOA2

Target: APOA2 (Apolipoprotein A-II (APOA2))

Andere Bezeichnung: Apolipoprotein A II (Apo AII)

Hintergrund: Apolipoprotein A II is the second most abundant protein of the high density lipoprotein particles. The apolipoprotein A II gene consists of 4 exons and 3 introns. The four exons encode the 5' untranslated region, pre peptide, a short N terminal domain and a C terminal domain composed of a variable number of lipid binding amphipathic helices. Familial apolipoprotein A II deficiency may result from a splice junction alteration which blocks splicing of intron 3 from the primary transcript and result in the formation of a non functional mRNA.Synonyms: APOA2, Apo-AII, ApoA-II, Apolipoprotein A-II, Apolipoprotein A2

Gen-ID: 336

NCBI Accession: NP_001634

UniProt: P02652

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Negative Regulation of Transporter Activity, Lipid Metabolism