CXCL12 Antikörper (Biotin)

Produktnummer ABIN116455

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch CXCL12 in WB und EIA. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für CXCL12 Antikörper (Biotin) (ABIN116455)

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CXCL12 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-CXCL12 Antikörper (Biotin)

Spezifität: Reacts with Mouse Stromal Cell Derived Factor-1 alpha (SDF-1 alpha). Other species not tested.

Aufreinigung: Affinity Chromatography.

Immunogen: Highly pure (>98%) recombinant Mouse SDF-1 alpha. Remarks: Molecular Weight: ~150 kDa

Anwendungsinformationen

Applikationshinweise: Direct ELISA: To detect Mouse SDF-1 alpha by direct ELISA (using 100 μL/well antibodysolution) this antibody can be used at a concentration of 0.15-0.30 μg/mL. Used inconjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant Mouse SDF-1 alpha. Sandwich ELISA: To detect Mouse SDF-1 alpha by sandwich ELISA (using 100 μL/wellantibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. Thisbiotinylated polyclonal antibody, in conjunction with Anti-Mouse SDF-1 alpha (PP1217P1 orPP1217P2) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant MOuse SDF-1 alpha. Western Blot: To detect Mouse SDF-1alpha by Western Blot analysis this antibody can beused at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Mouse SDF-1 alpha is 1.5-3.0 ng/lane, undereither reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu CXCL12

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Andere Bezeichnung: CXCL12 / SDF1

Hintergrund: SDF1 alpha and 1 beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF or IL1. SDF1 alpha has been shown to be chemotactic for lymphocytes and is recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV1 entry.Synonyms: C-X-C motif chemokine 12, IRH, Intercrine reduced in hepatomas, PBSF, Pre-B cell growth-stimulating factor, SDF1A, SDF1B, Stromal cell-derived factor 1, hIRH

Molekulargewicht: approx. 150 kDa

Gen-ID: 20315

NCBI Accession: NP_001012495

UniProt: P40224

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling