IL-13 Antikörper

Produktnummer ABIN116389

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch IL-13 in WB, Func und EIA. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für IL-13 Antikörper (ABIN116389)

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL-13 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Functional Studies (Func), Enzyme Immunoassay (EIA)

Produktdetails anti-IL-13 Antikörper

Spezifität: Reacts with Murine Interleukin 13.

Aufreinigung: Affinity chromatography.

Immunogen: Highly purified (>98%) recombinant mouse IL-13

Anwendungsinformationen

Applikationshinweise: Neutralization. ELISA: (Indirect): To detect mIL-13 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant mIL-13. (Sandwich): To detect mIL-13 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Details zu IL-13

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Andere Bezeichnung: Interleukin-13 / IL13

Hintergrund: Human IL13 was originally identified by differential screening of an anti CD28 activated human peripheral blood mononuclear cell cDNA library as an induction specific novel cytokine. It was also isolated from cDNA libraries of human T cell clones using the murine IL13 (P600) cDNA as a probe. Human IL13, a pleiotropic cytokine, is produced by activated Th0, Th1 like, Th2 like, and CD8 T cells. The gene for human IL13 maps to chromosome 5 and is closely linked to the genes for IL3, IL4, IL5, and GMCSF. IL13 inhibits proinflammatory cytokine production and stimulates antibody production. It induces proliferation in the human pre myeloid cell line TF1. IL13 has multiple effects on the differentiation and functions of monocytes and macrophages. It suppresses cytotoxic functions and induces changes in the morphology of human monocytes and in the phenotype of human monocytes and B cells by upregulating MHC class II expression. IL13 will also decrease the production of nitric oxide by activated murine macrophages, leading to impaired parasiticidal activity. Human and mouse interleukin 13 share approximately 58 % amino acid sequence identity. Although human and mouse IL13 are equally active on human cells, human IL13 is much less active than mouse IL13 on mouse cells. Human IL13 and human IL4 also share approximately 30 % sequence homology and have similar biological functions.Synonyms: IL-13, NC30

Molekulargewicht: 150 kDa

Gen-ID: 16163

NCBI Accession: NP_032381

UniProt: P20109

Pathways: JAK-STAT Signalweg, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport