beta 2 Defensin Antikörper (Biotin)

Produktnummer ABIN116359

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch beta 2 Defensin in WB und EIA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für beta 2 Defensin Antikörper (Biotin) (ABIN116359)

Target: beta 2 Defensin (BD-2) (Defensin beta 2 (BD-2))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser beta 2 Defensin Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-beta 2 Defensin Antikörper (Biotin)

Spezifität: This antibody recognizes Human BD2 (Defensin beta 2). Other species not tested.

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: Highly pure (>98%) recombinant Human BD-2 (hBD-2)

Anwendungsinformationen

Applikationshinweise: Direct ELISA: To detect hBD-2 by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hBD-2. Sandwich ELISA: To detect hBD-2 by Sandwich ELISA (using 100 μL/well antibody solution)a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with a compatible polyclonal Anti-Human BD-2 (PP1125P1 orPP1125P2) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant hBD-2. Western Blot: To detect hBD-2 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hBD-2 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized antibody is stable at -20 °C for one year from date of despatch. The reconstituted solution can be stored at 2-8 °C for two weeks. Frozen aliquots of this solution are stable for six months when kept at -20 °C.

Haltbarkeit: 12 months

Details zu beta 2 Defensin

Target: beta 2 Defensin (BD-2) (Defensin beta 2 (BD-2))

Andere Bezeichnung: Defensin beta 2

Hintergrund: This antibiotic peptide is locally regulated by inflammation. Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. hBD-2 is a antimicrobial peptide, which belongs to the distinct family of beta-defensins.Synonyms: BD-2, Beta-defensin 2, Beta-defensin 4A, DEFB102, DEFB2, DEFB4A, DEFB4B, Skin-antimicrobial peptide 1, hBD-2

Gen-ID: 100289462

NCBI Accession: NP_001192195

UniProt: O15263