Thrombopoietin Antikörper

Produktnummer ABIN116255

Der Ziege Polyklonal anti-Thrombopoietin Antikörper wird verwendet zum Nachweis von Thrombopoietin in Proben von Human. Er wurde validiert für WB, EIA und Func.

Kurzübersicht für Thrombopoietin Antikörper (ABIN116255)

Target: Thrombopoietin (THPO)

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Thrombopoietin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Produktdetails anti-Thrombopoietin Antikörper

Spezifität: This antibody reacts with Thrombopoietin.

Aufreinigung: Affinity chromatography.

Immunogen: Highly purified recombinant human TPO.

Anwendungsinformationen

Applikationshinweise: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhTPO (5.00 ng/mL), a concentration of 4.2-6.3 μg/mL of this antibody is required. ELISA: (Indirect): To detect hTPO by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hTPO. (Sandwich): To detect hTPO by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human TPO (PP1074B) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTPO. Western Blot: To detect hTPO by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTPO is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu Thrombopoietin

Target: Thrombopoietin (THPO)

Andere Bezeichnung: Thrombopoietin

Hintergrund: Synonyms: C-mpl ligand, MGDF, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, THPO

Gen-ID: 7066

NCBI Accession: NP_000451

UniProt: P40225

Pathways: JAK-STAT Signalweg, Hormone Activity