ELISA: Direct: To detect hTARC by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hTARC. Sandwich: To detect hTARC by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with Polyclonal Anti-Human TARC (PP1069P) as a captureantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTARC. Western Blot: To detect hTARC by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTARC is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2 without preservatives.
Konservierungsmittel
Without preservative
Handhabung
Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody at 2-8 °C for one month or at -20 °C for longer.
Target
CCL17
(Chemokine (C-C Motif) Ligand 17 (CCL17))
Andere Bezeichnung
TARC / CCL17
Hintergrund
Synonyms: C-C motif chemokine 17, CC chemokine TARC, SCYA17, Small-inducible cytokine A17, Thymus and activation-regulated chemokine