CXCL12 Antikörper (Biotin)

Produktnummer ABIN116227

Produktdetails anti-CXCL12 Antikörper (Biotin)

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CXCL12 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: Recognizes Stromal Cell Derived Factor-1 alpha (SDF-1 alpha).

Aufreinigung: Affinity Chromatography.

Immunogen: Highly pure (>98%) E.coli derived recombinant Human SDF1-alpha.

Anwendungsinformationen

Applikationshinweise: ELISA: To detect hSDF-1alpha by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. This antibody, in conjunctionwith compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hSDF-1alpha. To detect hSDF-1alpha by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. This antibody, in conjunctionwith a polyclonal Anti-Human SDF-1alpha (PP1067P) as a capture antibody, allows thedetection of at least 0.2 - 0.4 ng/well of recombinant hSDF-1alpha. Western Blot: To detect hSDF-1alpha by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hSDF-1alpha is 1.5 - 3.0 ng/lane, under either reducingor non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Referenzen für anti-CXCL12 Antikörper (Biotin) (ABIN116227)

Schlaepfer, Audigé, Joller, Speck: "TLR7/8 triggering exerts opposing effects in acute versus latent HIV infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 5, pp. 2888-95, (2006) (PubMed).

Details zu CXCL12

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Andere Bezeichnung: CXCL12 / SDF1 (CXCL12 Produkte)

Synonyme: IRH antikoerper, PBSF antikoerper, SCYB12 antikoerper, SDF1 antikoerper, TLSF antikoerper, TPAR1 antikoerper, CINC-1 antikoerper, Gro1 antikoerper, Pbsf antikoerper, Scyb12 antikoerper, Sdf1 antikoerper, Tlsf antikoerper, Tpar1 antikoerper, Fsp antikoerper, KC antikoerper, Mgsa antikoerper, N51 antikoerper, Scyb1 antikoerper, gro antikoerper, sdf-1 antikoerper, sdf1 antikoerper, xSDF-1 antikoerper, SDF-1 antikoerper, cxcl12 antikoerper, sdf-1a antikoerper, sdf1a antikoerper, wu:fa55e10 antikoerper, wu:fc16h12 antikoerper, wu:fj84c02 antikoerper, C-X-C motif chemokine ligand 12 antikoerper, C-X-C motif chemokine ligand 1 antikoerper, chemokine (C-X-C motif) ligand 12 antikoerper, chemokine (C-X-C motif) ligand 1 antikoerper, C-X-C motif chemokine ligand 12 L homeolog antikoerper, chemokine (C-X-C motif) ligand 12a (stromal cell-derived factor 1) antikoerper, CXCL12 antikoerper, cxcl12 antikoerper, Cxcl1 antikoerper, Cxcl12 antikoerper, cxcl12.L antikoerper, cxcl12a antikoerper

Hintergrund: SDF1 alpha and 1 beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF or IL1. SDF1 alpha has been shown to be chemotactic for lymphocytes and is recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV1 entry.Synonyms: C-X-C motif chemokine 12, IRH, Intercrine reduced in hepatomas, PBSF, Pre-B cell growth-stimulating factor, SDF1A, SDF1B, Stromal cell-derived factor 1, hIRH

Gen-ID: 6387

NCBI Accession: NP_000600

UniProt: P48061

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling