Oncostatin M Antikörper

Produktnummer ABIN116203

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Oncostatin M in WB, IHC (p), EIA und Func. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Oncostatin M Antikörper (ABIN116203)

Target: Oncostatin M (OSM)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Oncostatin M Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Produktdetails anti-Oncostatin M Antikörper

Spezifität: Human Oncostatin M.

Aufreinigung: Affinity Chromatography employing immobilized Human Oncostatin M matrix

Immunogen: Highly pure (>98%) E.coli derived recombinant Human Oncostatin M.

Anwendungsinformationen

Applikationshinweise: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhuman Oncostatin M (10 ng/mL), a concentration of 0.1 - 0.175 μg/mL of this antibody isrequired. Immunohistochemistry: We recommend use of 1 g per ml as a starting dilution. Paraffinsections may require pretreatment like Proteinase K or Trypsin predigestion to improvestaining. ELISA: To detect human Oncostatin M by direct ELISA (using 100 l/well antibody solution)a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant human Oncostatin M. Western Blot: use a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatiblesecondary reagents the detection limit for recombinant human Oncostatin M is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Details zu Oncostatin M

Target: Oncostatin M (OSM)

Andere Bezeichnung: Oncostatin-M

Hintergrund: Synonyms: OSM

Molekulargewicht: approx. 150 KDa

Gen-ID: 5008

NCBI Accession: NP_065391

UniProt: P13725

Pathways: JAK-STAT Signalweg, Negative Regulation of Hormone Secretion