CCL19 Antikörper

Produktnummer ABIN116176

Produktdetails anti-CCL19 Antikörper

Target: CCL19 (Chemokine (C-C Motif) Ligand 19 (CCL19))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CCL19 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Spezifität: This antibody reacts with human Macrophage Inflammatory Protein-3 beta (hMIP-3 beta).

Aufreinigung: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hMIP-3 beta.

Anwendungsinformationen

Applikationshinweise: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhMIP-3 beta (100 ng/mL), a concentration of 2.7 - 4.0 μg/mL of this antibody is required. ELISA: To detect hMIP-3 beta by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hMIP-3 beta. Western Blot: To detect hMIP-3 beta by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMIP-3 beta is 1.5 - 3.0 ng/lane, under either reducingor non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu CCL19

Target: CCL19 (Chemokine (C-C Motif) Ligand 19 (CCL19))

Andere Bezeichnung: MIP3-beta / CCL19 (CCL19 Produkte)

Synonyme: CKb11 antikoerper, ELC antikoerper, MIP-3b antikoerper, MIP3B antikoerper, SCYA19 antikoerper, CCL19 antikoerper, Scya19 antikoerper, exodus-3 antikoerper, C-C motif chemokine ligand 19 antikoerper, chemokine (C-C motif) ligand 19 antikoerper, CCL19 antikoerper, ccl19 antikoerper, Ccl19 antikoerper

Hintergrund: MIP3 chemokines are among many novel beta chemokines recently identified through bioinformatics in the Expressed Sequence Tag (EST) database. They are distantly related to other beta chemokines (20-30 % aa sequence homology). MIP3 chemokines are useful probes for studying the biology of inflammation and cell migration. Their expression is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL10. MIP3 beta is also known as ELC (EBI1-1 ligand chemokine) and is constitutively expressed in various lymphoid tissues (thymus, lymph nodes, appendix and spleen). MIP3 beta is a chemoattractant for cultured human lymphocytes, dendritic cells, human T lymphoblastoid CEM NKR2 and hemopoietic progenitor cells. It may be involved in G Protein activation in human NK cells. MIP3 beta is a unique functional ligand for CCR7. The MIP3 beta gene has been mapped to chormosome 9p13.Synonyms: Beta chemokine exodus-3, C-C motif chemokine 19, CCL-19, CK beta-11, EBI1-ligand chemokine, ELC, Exodus-3, MIP-3 beta, MIP3B, Macrophage inflammatory protein 3 beta, SCYA19, Small-inducible cytokine A19, Small-inducible cytokine A19

Gen-ID: 6363

NCBI Accession: NP_006265

UniProt: Q99731

Pathways: Positive Regulation of Immune Effector Process