FGF7 Antikörper

Produktnummer ABIN116127

Dieses Anti-FGF7-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von FGF7 in WB, IHC (p), EIA und Func. Geeignet für Human. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für FGF7 Antikörper (ABIN116127)

Target: FGF7 (Fibroblast Growth Factor 7 (FGF7))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FGF7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Produktdetails anti-FGF7 Antikörper

Spezifität: This antibody reacts with Human Keratinocyte Growth Factor (KGF).

Aufreinigung: Affinity chromatography

Immunogen: Highly purified (>98%) recombinant FGF-7 (KGF)

Anwendungsinformationen

Applikationshinweise: Neutralisation: To yield one-half maximal inhibition [ND50]of the biological activity of hKGF(25 ng/mL), a concentration of 0.56-0.85 μg/mL of this antibody has been used for previouslots. ELISA: To detect hKGF by direct ELISA (using 100 l/well antibody solution) a concentrationof at least 0.5g/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/wellof recombinant hKGF. Western blot: To detect hKGF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hKGF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Referenzen für anti-FGF7 Antikörper (ABIN116127)

Lim, Poh, Riau, Beuerman, Tan, Mehta: "Biological and ultrastructural properties of acelagraft, a freeze-dried γ-irradiated human amniotic membrane." in: Archives of ophthalmology, Vol. 128, Issue 10, pp. 1303-10, (2010) (PubMed).

Details zu FGF7

Target: FGF7 (Fibroblast Growth Factor 7 (FGF7))

Andere Bezeichnung: FGF7 / KGF

Hintergrund: KGF is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.Synonyms: Fibroblast growth factor 7, HBGF7, Heparin-binding growth factor 7, Keratinocyte growth factor

Gen-ID: 2252

NCBI Accession: NP_002000

UniProt: P21781

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung