IL-5 Antikörper (Biotin)

Produktnummer ABIN116077

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch IL-5 in WB und EIA. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für IL-5 Antikörper (Biotin) (ABIN116077)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL-5 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-IL-5 Antikörper (Biotin)

Spezifität: This antibody recognizes Human IL-5.

Aufreinigung: Affinity Chromatography.

Immunogen: Highly pure (>98%) E.coli derived recombinant hIL-5 (human IL-5).

Anwendungsinformationen

Applikationshinweise: ELISA: To detect hIL-5 by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15-0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hIL-5. Western Blot: To detect hIL-5 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-5 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Centrifuge vial prior to opening! This product is photosensitive and should be protected from light

Lagerung: 4 °C

Informationen zur Lagerung: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Details zu IL-5

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Andere Bezeichnung: Interleukin-5 / IL5

Hintergrund: Interleukin 5 (IL5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL2, IL4 and growth hormone. Human and mouse IL5 have 70 % amino acid sequence homology.Synonyms: B-cell differentiation factor I, Eosinophil differentiation factor, IL-5, T-cell replacing factor, TRF

Gen-ID: 3567

NCBI Accession: NP_000870

UniProt: P05113

Pathways: JAK-STAT Signalweg, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour