G-CSF Antikörper (Biotin)

Produktnummer ABIN116023

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch G-CSF in WB und EIA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für G-CSF Antikörper (Biotin) (ABIN116023)

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser G-CSF Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-G-CSF Antikörper (Biotin)

Spezifität: Human Granulocyte Colony Stimulating Factor (G-CSF).

Aufreinigung: Affinity Chromatography employing immobilized hG-CSF matrix.

Immunogen: Highly pure (>98%) recombinant hG-CSF (human G-CSF)

Anwendungsinformationen

Applikationshinweise: ELISA: (Direct): To detect hG-CSF by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hG-CSF. (Sandwich): To detect hG-CSF by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with Polyclonal Anti-Human G-CSF (PP1011P1 or PP1011P2) as acapture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hG-CSF. Western Blot: To detect hG-CSF by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hG-CSF is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Centrifuge vial prior to opening! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody at 2-8 °C. DO NOT FREEZE!

Details zu G-CSF

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Andere Bezeichnung: G-CSF

Hintergrund: Granulocyte Colony Stimulating Factor (G-CSF) a cytokine that controls the production, differentiation, and function of granulocytes. It is a potent stimulator of bone marrow cells especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils.Synonyms: C17orf33, CSF3, Colony stimulating factor 3, GCSF, Granulocyte colony-stimulating factor, Pluripoietin

Gen-ID: 1440

NCBI Accession: NP_000750

UniProt: P09919

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization