CX3CL1 Antikörper

Produktnummer ABIN116017

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch CX3CL1 in WB, IHC (p), EIA und Func. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für CX3CL1 Antikörper (ABIN116017)

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CX3CL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Produktdetails anti-CX3CL1 Antikörper

Spezifität: This antibody is specific to Human Fractalkine.

Aufreinigung: Affinity Chromatography

Immunogen: Highly pure (> 98%) E.coli derived recombinant Human Fractalkine (Cat.-No PA057). Remarks: Molecular Weight: ~150 kDa

Anwendungsinformationen

Applikationshinweise: Neutralization. Sandwich ELISA: To detect Human Fractalkine by Sandwich ELISA (using 100 μL/wellantibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. Thisantigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Fractalkine(PP1009B1 or PP1009B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Fractalkine. Western Blot: To detect Human Fractalkine by Western Blot analysis this antibody can beused at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hFractalkine is 1.5-3.0 ng/lane, under eitherreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Details zu CX3CL1

Target: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Andere Bezeichnung: Fractalkine / CX3CL1

Hintergrund: CX3CL1 is a member of the delta chemokine subfamily that contains a unique CX3C cysteine motif near the N-terminal. Unlike other known chemokines, it is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. CX3CL1, a leukocyte chemoattractant, is expressed in various tissues including the brain, heart, lung, kidney, skeletal muscle, and testis. The expression is reported to be up-regulated in endothelial cells and microglia by inflammatory signals. CX3CR1, a specific receptor for fractalkine, mediates both leukocyte migration and adhesion.Synonyms: C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, FKN, NTT, Neurotactin, SCYD1, Small-inducible cytokine D1

Gen-ID: 6376

NCBI Accession: NP_002987

UniProt: P78423

Pathways: Synaptic Membrane