CX3CL1 Antikörper (Biotin)
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- Target Alle CX3CL1 Antikörper anzeigen
- CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser CX3CL1 Antikörper ist konjugiert mit Biotin
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Applikation
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Spezifität
- Human Fractalkine.
- Aufreinigung
- Affinity chromatography
- Immunogen
- Highly pure (> 98%) recombinant hFractalkine (human Fractalkine)
- Top Product
- Discover our top product CX3CL1 Primärantikörper
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- Applikationshinweise
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ELISA: (Direct): To detect hFractalkine by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hFractalkine. (Sandwich): To detect hFractalkine by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylatedpolyclonal antibody, in conjunction with Polyclonal Anti-Human Fractalkine (PP1009P) as acapture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinanthFractalkine. Western Blot: To detect hFractalkine by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFractalkine is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Rekonstitution
- Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
- Buffer
- PBS, pH 7.2, without preservatives
- Konservierungsmittel
- Without preservative
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- The lyophilized antibody is stable at -20 °C for one year from despatch. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.
- Haltbarkeit
- 12 months
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- Target
- CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))
- Andere Bezeichnung
- Fractalkine / CX3CL1 (CX3CL1 Produkte)
- Synonyme
- ABCD3 antikoerper, CX3CL1 antikoerper, DKFZp459K117 antikoerper, Cx3c antikoerper, Scyd1 antikoerper, ABCD-3 antikoerper, C3Xkine antikoerper, CXC3 antikoerper, CXC3C antikoerper, NTN antikoerper, NTT antikoerper, SCYD1 antikoerper, fractalkine antikoerper, neurotactin antikoerper, AB030188 antikoerper, AI848747 antikoerper, CX3C antikoerper, Cxc3 antikoerper, D8Bwg0439e antikoerper, ATP binding cassette subfamily D member 3 antikoerper, C-X3-C motif chemokine ligand 1 antikoerper, chemokine (C-X3-C motif) ligand 1 antikoerper, ABCD3 antikoerper, CX3CL1 antikoerper, Cx3cl1 antikoerper, Abcd3 antikoerper
- Hintergrund
- CX3CL1 is a member of the delta chemokine subfamily that contains a unique CX3C cysteine motif near the N-terminal. Unlike other known chemokines, it is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. CX3CL1, a leukocyte chemoattractant, is expressed in various tissues including the brain, heart, lung, kidney, skeletal muscle, and testis. The expression is reported to be up-regulated in endothelial cells and microglia by inflammatory signals. CX3CR1, a specific receptor for fractalkine, mediates both leukocyte migration and adhesion.Synonyms: C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, FKN, NTT, Neurotactin, SCYD1, Small-inducible cytokine D1
- Gen-ID
- 6376
- NCBI Accession
- NP_002987
- UniProt
- P78423
- Pathways
- Synaptic Membrane
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