FGF1 Antikörper

Produktnummer ABIN116005

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch FGF1 in WB, IHC (p), EIA und Func. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für FGF1 Antikörper (ABIN116005)

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FGF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Produktdetails anti-FGF1 Antikörper

Spezifität: Human Fibroblast Growth Factor acidic (hFGF-1)

Aufreinigung: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hFGF-acidic.

Anwendungsinformationen

Applikationshinweise: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhFGF-acidic (30 ng/mL), a concentration of 0.46 - 0.9 μg/mL of this antibody is required. ELISA: To detect hFGF-acidic by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant hFGF-acidic. Western Blot: To detect hFGF-acidic by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFGF-acidic is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Centrifuge vial prior to opening!

Lagerung: 4 °C

Details zu FGF1

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Andere Bezeichnung: FGF Acidic / FGF1

Hintergrund: The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these two growth factors.Synonyms: Acidic fibroblast growth factor, Beta-endothelial cell growth factor, ECGF-beta, FGFA, HBGF-1, HBGF1, Heparin-binding growth factor 1

Gen-ID: 2246

NCBI Accession: NP_000791

UniProt: P05230

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung