Cardiotrophin 1 Antikörper

Produktnummer ABIN115989

Dieses Anti-Cardiotrophin 1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Cardiotrophin 1 in WB, EIA und Func. Geeignet für Human.

Kurzübersicht für Cardiotrophin 1 Antikörper (ABIN115989)

Target: Cardiotrophin 1 (CTF1)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cardiotrophin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Produktdetails anti-Cardiotrophin 1 Antikörper

Spezifität: This antibody reacts with Human Cardiotrophin-1

Aufreinigung: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hCardiotrophin-1.

Anwendungsinformationen

Applikationshinweise: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhCardiotrophin-1 (3 ng/mL), a concentration of 0.04-0.09 μg/mL of this antibody isrequired. ELISA: To detect hCardiotrophin-1 by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hCardiotrophin-1. Western Blot: To detect hCardiotrophin-1 by Western Blot analysis this antibody can beused at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hCardiotrophin-1 is 1.5-3.0 ng/lane, underreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu Cardiotrophin 1

Target: Cardiotrophin 1 (CTF1)

Andere Bezeichnung: Cardiotrophin-1

Hintergrund: Cardiotrophin-1 (CT-1), a cardiac hypertrophic factor, is a 21.5 kDa protein member of the IL-6 cytokine family. CT-1 is associated with the pathophysiology of heart diseases, including hypertension, myocardial infarction, valvular heart disease, and congestive heart failure. The protein exerts its cellular effects by interacting with the glycoprotein 130 (gp130)/leukemia inhibitory factor receptor beta (LIFR) heterodimer. In addition, CT-1 activates phosphatidylinositol 3-kinase (PI-3 kinase) in cardiac myocytes and enhances transcription factor NF kappa B DNA -binding activities. CT-1 is highly expressed in the heart, skeletal muscle, prostate and ovary and to lower levels in lung, kidney, pancreas, thymus, testis and small intestine.Synonyms: CT-1, CTF1

Gen-ID: 1489

NCBI Accession: NP_001136016

UniProt: Q16619

Pathways: JAK-STAT Signalweg