BDNF Antikörper (Biotin)

Produktnummer ABIN115983

Produktdetails anti-BDNF Antikörper (Biotin)

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BDNF Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: Recognizes Human BDNF.

Kreuzreaktivität (Details): Species reactivity (tested):Human

Aufreinigung: Affinity chromatography

Immunogen: Highly purified (>98%) recombinant Human BDNF

Anwendungsinformationen

Applikationshinweise: ELISA: Direct: To detect Human BDNF by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant Human BDNF. Sandwich: To detect Human BDNF by Sandwich ELISA (using 100 μL/well antibody solution)a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with Anti-Human BDNF (PP1000P1 or PP1000P1) as a captureantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human BDNF. Western blot: To detect Human BDNF by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Referenzen für anti-BDNF Antikörper (Biotin) (ABIN115983)

Hartog, Dittrich, Pieneman, Jansen, Frankl-Vilches, Lessmann, Lilliehook, Goldman, Gahr: "Brain-derived neurotrophic factor signaling in the HVC is required for testosterone-induced song of female canaries." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 49, pp. 15511-9, (2009) (PubMed).

Details zu BDNF

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Andere Bezeichnung: BDNF (BDNF Produkte)

Synonyme: ANON2 antikoerper, BULN2 antikoerper, bdnf-A antikoerper, BDNF antikoerper, bdnf antikoerper, brain derived neurotrophic factor antikoerper, brain-derived neurotrophic factor antikoerper, brain-derived neurotrophic factor L homeolog antikoerper, brain-derived neurotrophic factor S homeolog antikoerper, BDNF antikoerper, Bdnf antikoerper, bdnf antikoerper, bdnf.L antikoerper, bdnf.S antikoerper

Hintergrund: Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes NGF, NT3, and NT4. All neurotrophins have six conserved cysteine residues and share a 55 % sequence identity at the amino acid level. BDNF is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells. BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non neuronal cells. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients. In addition, functional studies showed that age-associated changes in BDNF-mediated pathways can enhance inflammation and increase myocardial injury after myocardial infarction in the aging heart.Synonyms: Abrineurin, Brain-derived neurotrophic factor

Gen-ID: 627

NCBI Accession: NP_001137277

UniProt: P23560

Pathways: RTK Signalweg, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity