CCL3 Antikörper (Biotin)

Produktnummer ABIN115967

Der Kaninchen Polyklonal Anti-CCL3-Antikörper wurde für WB und EIA validiert. Er ist geeignet, CCL3 in Proben von Ratte zu detektieren.

Kurzübersicht für CCL3 Antikörper (Biotin) (ABIN115967)

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CCL3 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-CCL3 Antikörper (Biotin)

Spezifität: Recognises Rat MIP-1 alpha.

Aufreinigung: Affinity Chromatography employing an immobilized Rat MIP-1-alpha matrix

Immunogen: Highly pure (>98%) recombinant Rat MIP-1-alpha (Rat MIP-1-alpha).

Anwendungsinformationen

Applikationshinweise: ELISA: To detect rat MIP-1-alpha by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15-0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinantrat MIP-1-alpha. Western Blot: To detect rat MIP-1-alpha by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant rat MIP-1-alpha is 1.5 - 3.0 ng/lane, under eitherreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Details zu CCL3

Target: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Andere Bezeichnung: MIP1 alpha / CCL3

Hintergrund: Macrophage Inflammatory Protein 1 alpha (MIP1 alpha) and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77 % and 70 % amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. Has adhesive effects on lymphocytes. MIP1 alpha can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. A signal transducing receptor, designated the C-C chemokine receptor-1 (C-C CKR-1) with seven transmembrane domains that bind MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities, has been isolated.Synonyms: C-C motif chemokine 3, CCL-3, G0/G1 switch regulatory protein 19-1, G0S19-1 protein, LD78 alpha, MIP-1-alpha, MIP1A, Macrophage Inflammatory Protein 1 alpha, SCYA3, Small-inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein

Gen-ID: 25542

NCBI Accession: NP_037157

UniProt: P50229

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagie