CNTF Antikörper (Biotin)

Produktnummer ABIN115935

Dieses Anti-CNTF-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CNTF in WB und EIA. Geeignet für Ratte.

Kurzübersicht für CNTF Antikörper (Biotin) (ABIN115935)

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CNTF Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-CNTF Antikörper (Biotin)

Spezifität: This antibody recognises Rat CNTF.

Aufreinigung: Affinity Chromatography.

Immunogen: Highly purified recombinant CNTF.

Anwendungsinformationen

Applikationshinweise: ELISADirect: To detect Rat CNTF by indirect ELISA (using 100 ml/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant Rat CNTF. Sandwich: To detect Rat CNTF by sandwich ELISA (using 100 ml/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. The Biotinylated antibody inconjunction with Anti-Rat CNTF (PP030P1 or PP030P2) as a Capture antibody, allows thedetection of at least 0.2-0.4 ng/well of recombinant Rat CNTF. Western BlotTo detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu CNTF

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Andere Bezeichnung: CNTF

Hintergrund: CNTF is a potent neural factor that was originally characterized as a vital factor for the survival of chick ciliary neurons in vitro. CNTF is also important for the survival of other neural cell types including primary sensory neurons, motor neurons, basal forebrain neurons and type 2 astrocytes. CNTF is highly conserved across species and exhibits cross-species bioactivity.Synonyms: Ciliary Neurotrophic Factor

Gen-ID: 25707

NCBI Accession: NP_037298

UniProt: P20294

Pathways: JAK-STAT Signalweg