GM-CSF Antikörper

Produktnummer ABIN115853

Der Kaninchen Polyklonal Anti-GM-CSF-Antikörper wurde für WB, EIA und Func validiert. Er ist geeignet, GM-CSF in Proben von Maus zu detektieren.

Kurzübersicht für GM-CSF Antikörper (ABIN115853)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GM-CSF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Produktdetails anti-GM-CSF Antikörper

Spezifität: This antibody reacts with Mouse GM-CSF.

Aufreinigung: Affinity Chromatography employing immobilized mGM-CSF matrix.

Immunogen: E.coli derived Highly pure (>98%) recombinant Murine GM-CSF Remarks: Mol. Weight: ~150 kDa

Anwendungsinformationen

Applikationshinweise: NeutralizationTo yield one-half maximal inhibition [ND50] of the biological activity of mGM-CSF (0.60 ng/mL), a oncentration of 0.012-0.02 μg/mL of this antibody is required. ELISAIndirect: To detect Mouse GM-CSF by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant Mouse GM-CSF. Sandwich: To detect mGM-CSF by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Murine GM-CSF (PP003B1 or PP003B2) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinantmGM-CSF. Western Blot: To detect Mouse GM-CSF by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mGM-CSF is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Andere Bezeichnung: GM-CSF

Hintergrund: The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.Synonyms: CSF2, Colony stimulating factor 2, GMCSF, Granulocyte-macrophage colony-stimulating factor, Molgramostin, Sargramostim

Gen-ID: 12981

NCBI Accession: NP_034099

UniProt: P01587

Pathways: JAK-STAT Signalweg, Cellular Response to Molecule of Bacterial Origin