CCL8 Antikörper

Produktnummer ABIN115779

Der Maus Monoklonal anti-CCL8 Antikörper (ABIN115779) detektiert spezifisch CCL8 in WB, EIA und Func. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für CCL8 Antikörper (ABIN115779)

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CCL8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Produktdetails anti-CCL8 Antikörper

Spezifität: Reacts with Human Macrophage Chemotactic Protein-2 (MCP-2)

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Highly pure (>98%) recombinant Human MCP-2

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 1.0-2.0 μg/mL of thisantibody will detect at least 100 pg/mL of recombinant human MCP-2 when used withBiotin anti-Human MCP-2 antibody (cat. PP1047B) as the detection antibody at aconcentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect Human MCP-2 by Western Blot analysis this antibody can be usedat a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hMCP-2 is 0.25-0.50 ng/lane, undernon-reducing conditions. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman MCP-2 (100 ng/mL), a concentration of 0.8-1.0 μg/mL of this antibody is required.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu CCL8

Target: CCL8 (Chemokine (C-C Motif) Ligand 8 (CCL8))

Andere Bezeichnung: MCP2 / CCL8

Hintergrund: MCP 2 (Monocyte Chemoattractant Protein 2) is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. MCP 2 is important to inflammatory host responses, and is found in the highest concentration in the small intestine and peripheral blood cells. This protein is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection.Synonyms: C-C motif chemokine 8, CCL-8, HC14, MCP-2, Monocyte Chemotactic Protein-2, Monocyte chemoattractant protein 2, SCYA10, SCYA8, Small-inducible cytokine A8

Gen-ID: 6355

NCBI Accession: NP_005614

UniProt: P80075