IL-15 Antikörper

Produktnummer ABIN115774

Produktdetails anti-IL-15 Antikörper

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IL-15 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: Recognizes Human Interleukin-15 (IL-15).

Aufreinigung: Ammonium Sulfate Precipitation and Ion Exchange Chromatography

Immunogen: Recombinant hIL-15

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of thisantibody will detect at least 250 pg/mL of recombinant Human IL-15 when used with Biotinconjugated anti-Human IL-15 antibody (cat. PP1035B) as the detection antibody at aconcentration of approximately 2.0-4.0 μg/mL. Western Blot : To detect Human IL-15 by Western Blot analysis this antibody can be used ata concentration of 0.50-1.0 μg/mL (non-reducing) or at a concentration of 2.0-4.0 μg/mL(reducing). Used in conjunction with compatible secondary reagents the detection limit for

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu IL-15

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Andere Bezeichnung: Interleukin-15 / IL15 (IL15 Produkte)

Synonyme: IL15 antikoerper, AI503618 antikoerper, IL-15 antikoerper, interleukin 15 antikoerper, IL15 antikoerper, Il15 antikoerper

Hintergrund: IL15 (114 amino acids) is a cytokine that regulates T and natural killer cell activation and proliferation. It has a predicted molecular mass of approximately 12.5 kDa. Human IL15 shares approximately 97 % and 73 % amino acid sequence identity with simian and mouse IL15, respectively. Both human and simian IL15 are active on mouse cells. IL15 was initially isolated from the simian kidney epithelial cell line CV1/EBNA. It has also been isolated from mouse and human cell sources. The cytokines IL15 and IL2 share many biological properties and stimulatory activities (T, B, and NK cells). Both IL15 and IL2 stimulate mouse CTLL2 cells. In activated peripheral blood T lymphocytes, IL2 is highly expressed but the expression of IL15 is not detectable. There is no sequence homology between IL15 and IL2, though computer modeling indicates both possess a four alpha helical bundle structure. IL15 competes for binding sites with IL2, as both IL2 and IL15 stimulate the growth of cells through the IL2 receptor. IL15 mRNA is expressed in many cell types and tissues including adherent peripheral blood mononuclear cells, fibroblasts, and epithelial cells, monocytes, placenta, and skeletal muscle. IL-15 (14-15 kD) is a member of the four alpha-helical bundle family of cytokines. It is very similar to IL-2, except that IL-15 has an IL-15 alpha receptor subunit. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. This implies that IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases.Synonyms: IL-15

Gen-ID: 3600

NCBI Accession: NP_000576

UniProt: P40933

Pathways: JAK-STAT Signalweg, Glycosaminoglycan Metabolic Process