HGF Antikörper

Produktnummer ABIN115703

Der Kaninchen Polyklonal Anti-HGF-Antikörper wurde für WB und EIA validiert. Er ist geeignet, HGF in Proben von Human zu detektieren.

Kurzübersicht für HGF Antikörper (ABIN115703)

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HGF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-HGF Antikörper

Spezifität: The antibody recognizes human HGF. Other species not tested.

Aufreinigung: Protein A chromatography

Immunogen: Recombinant human HGF

Anwendungsinformationen

Applikationshinweise: ELISA: 1-15 μg/mL. Western blot: 1-2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water / PBS to a concentration of > 0.5 mg/mL.

Buffer: PBS, pH 7.4, without preservative or stabilizer

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing. When reconstituted to a concentration of > 0.5 mg/ml the antibody is stable for six weeks at 2-8°C.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized antibody can be stored at 2-8 °C for up to one month and at -20 °C for one year from despatch.

Haltbarkeit: 12 months

Details zu HGF

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Andere Bezeichnung: Hepatocyte Growth Factor / HGF

Hintergrund: Human Hepatocyte Growth Factor (HGF), also known as scatter factor, is a pleiotrophic cytokine that shows homology to the enzymes of the blood coagulation cascade. It stimulates the motility and invasion of several cancer cell types and can induce angiogenesis. Recently HGF was found to be identical to scatter factor, a fibroblast-derived factor promoting the dissociation of epithelial and vascular endothelial cell colonies in monolayer cell cultures by stimulating cell migration. HGF is synthesized as a biologically inactive single chain precursor, which is cleaved by a specific, extracellular serum serine protease to a fully active heterodimer. This mature, biologically active HGF consists of a disulfide-linked alpha-beta heterodimer of the two cleavage products. Previous studies have shown that single chain and heterodimeric HGF are equally active in in vitro assay systems due to either production of the serine protease in cell culture or the presence of the ubiquitious protease in serum. All biological responses induced by HGF are elicited by binding to its transmembrane tyrosine kinase receptor, which is encoded by the MET proto-oncogene. After autophosphorylation of the receptor different cytoplasmatic effectors are activated that bind to the same multifunctional docking site of the receptor. HGF function is essential for normal development. Hepatocytes have to be primed before they can fully respond to HGF. This priming requires cytokines as TNF and IL-6. Recent studies have suggested that HGF synergizes with basic FGF in the induction of angiogenesis.Synonyms: HPTA, Hepatopoeitin-A, Scatter factor

Gen-ID: 3082

NCBI Accession: NP_000592

UniProt: P14210

Pathways: RTK Signalweg, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor