His Tag Antikörper

Produktnummer ABIN115691

Der Kaninchen Polyklonal anti-His Tag Antikörper wird verwendet zum Nachweis von His Tag. Er wurde validiert für WB.

Kurzübersicht für His Tag Antikörper (ABIN115691)

Target: His Tag

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser His Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-His Tag Antikörper

Sequenz: HHHHHH

Aufreinigung: Antigen Affinity Chromatography using a His-peptide as matrix.

Immunogen: Different highly purified 6x His-tagged proteins (C-terminal) produced in insect cells.

Anwendungsinformationen

Applikationshinweise: Western blot (0.5-1 μg/mL): The antibody was tested using several His-tagged proteinsexpressed in insect cells and E. coli.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of > 0.5 mg/mL.

Buffer: PBS, pH 7.4, without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized IgG is stable at 2-8 °C for one month from despatch and for one year when kept at -20 °C. The reconstituted antibody can be stored at 2-8 °C for one month or at -20 °C for one year.

Haltbarkeit: 12 months

Details zu His Tag

Target: His Tag

Andere Bezeichnung: 6xHistidine Epitope Tag

Substanzklasse: Tag

Hintergrund: In the last couple of years many peptide sequences/epitopes for the purification of recombinant proteins have been established. These so-called