FGF2 Antikörper

Produktnummer ABIN115676

Der Kaninchen Polyklonal Anti-FGF2-Antikörper wurde für WB und Func validiert. Er ist geeignet, FGF2 in Proben von Human zu detektieren.

Kurzübersicht für FGF2 Antikörper (ABIN115676)

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FGF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Functional Studies (Func)

Produktdetails anti-FGF2 Antikörper

Spezifität: The antibody will detect FGF-2 (basic).

Aufreinigung: Protein A affinity chromatography

Immunogen: Highly pure (> 98%) recombinant human FGF-2 (basic)

Anwendungsinformationen

Applikationshinweise: Neutralization: To yield one-half maximal inhibition (ND50) of the biological activity of human FGF-2 basic(5 ng/mL) on HUVEC cells, a concentration of about 3.5 μg/mL of this antibody is required. Western Blot: To detect human FGF-2 basic by Western blot this antibody can be used at a concentrationof 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant human FGF-2 (basic) is 1.5 -3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of > / = 0.2 mg/mL.

Buffer: PBS, pH 7.4, without stabilizer or preservative

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized antibody is stable at room temperature for one month and for one year from despatch when kept at -20 °C. When reconstituted in sterile water to a concentration of > / = 0.2 mg/ml the antibody is stable for six weeks at 2-8 °C.

Haltbarkeit: 12 months

Details zu FGF2

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Andere Bezeichnung: FGF Basic / FGF2

Hintergrund: FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.Synonyms: BFGF, FGFB, Fibroblast growth factor 2 (basic), HBGF-2, HBGF2, Heparin-binding growth factor 2

Gen-ID: 2247

NCBI Accession: NP_001997

UniProt: P09038

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteine