ERCC1 Antikörper

Produktnummer ABIN115126

Produktdetails anti-ERCC1 Antikörper

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ERCC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Spezifität: This antibody reacts with a 33-36 kDa protein known as ERCC1 polypeptide. Cellular localization: Nuclear. Cross reacts with Rat.

Aufreinigung: Protein A Chromatography.

Immunogen: BALB/C mice were injected with full length recombinant human ERCC1 protein.

Klon: 8F1

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Immunoprecipitation. Immunohistochemistry on Paraffin Embedded Sections: 1/100-1/200 in an ABC method. Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to Immunostaining. Incubation Time: 30 minutes at RT. Recommended Positive Control: Tonsil. Western Blot: 1/100-1/500. use Human lung cell line as Positive Control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C.

Details zu ERCC1

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Andere Bezeichnung: ERCC1 (ERCC1 Produkte)

Synonyme: COFS4 antikoerper, RAD10 antikoerper, UV20 antikoerper, Ercc-1 antikoerper, ercc1 antikoerper, T12H1.18 antikoerper, T12H1_18 antikoerper, UV REPAIR DEFICIENT 7 antikoerper, UVR7 antikoerper, ERCC excision repair 1, endonuclease non-catalytic subunit antikoerper, excision repair cross-complementing rodent repair deficiency, complementation group 1 antikoerper, DNA excision repair protein ERCC-1 antikoerper, nucleotide repair protein antikoerper, excision repair cross-complementation group 1 L homeolog antikoerper, excision repair cross-complementation group 1 antikoerper, ERCC1 antikoerper, Ercc1 antikoerper, CC1G_04546 antikoerper, CpipJ_CPIJ013903 antikoerper, PITG_13719 antikoerper, ercc1 antikoerper, ercc1.L antikoerper

Hintergrund: The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1-XPF complex makes the 5' incision. Elevated levels of ERCC1 have also been reported in Cisplatin-resistant cells.Synonyms: COFS4, DNA excision repair protein ERCC-1, UV20

Gen-ID: 2067

NCBI Accession: NP_001974

UniProt: P07992

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response