Thrombospondin 1 Antikörper

Produktnummer ABIN115004

Der Maus Monoklonal anti-Thrombospondin 1 Antikörper wird verwendet zum Nachweis von Thrombospondin 1 in Proben von Säugetier. Er wurde validiert für WB, IF und IHC (p).

Kurzübersicht für Thrombospondin 1 Antikörper (ABIN115004)

Target: Thrombospondin 1 (THBS1)

Reaktivität: Säugetier

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Thrombospondin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: A6-1

Produktdetails anti-Thrombospondin 1 Antikörper

Spezifität: This antibody is specific to 450 kDa protein (non-reduced) and 170 to 180 kDa (reduced) form of Thrombospondin.
Cellular Localization: Secretory granules, Golgi complex, endoplasmic reticulum

Aufreinigung: Purified

Immunogen: BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunofluorescence. Immunoprecipitation. Western Blot. Immunohistochemistry on paraffin embedded sections: diluted of 1: 25-1: 50 in an ABC method (30 minutes at room temperature). Formalin fixedparaffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Sodium azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Thrombospondin 1

Target: Thrombospondin 1 (THBS1)

Andere Bezeichnung: Thrombospondin-1 (THBS1)

Hintergrund: Thrombospondin is a protein from platelet a granules. It is secreted at sites of platelet activation and aggregation and is involved in the differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.Synonyms: TSP, TSP1

Gen-ID: 7057

NCBI Accession: NP_003237

UniProt: P07996

Pathways: Autophagie