HSPG2 Antikörper

Produktnummer ABIN114947

Produktdetails anti-HSPG2 Antikörper

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Reaktivität: Human, Maus, Rind (Kuh), Ratte, Bakterien, Kabeljau, Wolf

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser HSPG2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: A7L6 recognizes domain IV of the core protein of the large Heparan Sulphate Proteoglycan or Perlecan. The reactivity is independent of the galactosaminoglycan moieties. Therefore, the epitope is not sensitive to Heparitinase treatment.

Aufreinigung: Purified

Immunogen: High molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG.

Klon: A7L6

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunoprecipitation. Immunoblotting: 1/100-1/1000. Immunocytochemistry. Immunohistochemistry on frozen and paraffin-embedded tissues: 1/25-1/200 (withavidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Do not freeze working dilutions.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the original vial at 2-8 °C. After reconstitution store (in aliquots) at -20 °C.

Details zu HSPG2

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Andere Bezeichnung: HSPG2 / Perlecan (HSPG2 Produkte)

Hintergrund: Proteoglycans are macromolecules consisting of a variety of core proteins with covalently attached one or several polysaccharide chains of the glycosaminoglycan type (heparan sulphate, heparin, chondroitin sulphate, dermatan sulphate or keratan sulphate). At least two forms of basement membrane heparan sulphate proteoglycan (HSPG) have been identified. One with a large core protein (> 400 kD) and one with a small core protein (30 kD). The large HSPG is probably the most abundant basement membrane proteoglycan. It is located predominantly in the lamina lucida, where it forms clustered aggregates and interacts with other basement membrane components to form the matrix. In addition, it also plays a critical role in attachment of cells to the basal membrane via integrin receptors.Synonyms: Basement membrane-specific heparan sulfate proteoglycan core protein, PLC

Gen-ID: 3339

NCBI Accession: NP_005520

UniProt: P98160

Pathways: Glycosaminoglycan Metabolic Process, Lipid Metabolism